the study of proteomic responses of hordeum bulbosom to salinity stress
نویسندگان
چکیده
a proteomic approach was conducted to further understand the mechanism of plant responses to salinity in hordeum bulbosom. three-week-old seedlings were treated with 300 mm of nacl for 21 days. total proteins of fourth leaf were extracted and separated by two-dimensional gel electrophoresis. more than 500 protein spots were reproducibly detected, including 46 spots that were up-regulated and 16 spots down-regulated. using maldi-tof-tof ms, 20 proteins involved in many cellular functions were identified. these proteins include: oxygen-evolving enhancer protein, rubisco, putative glycine decarboxylase, ribosomal protein, profilin, germin-like protein or oxalate oxidase-like protein, proxyredoxin, translationally-controlled tumor protein homology, nucleoside diphosphate kinase (ndpk), thioredoxin, dehydroascorbate reductase, 2-cys peroxiredoxin, replication factor c, and sucrose synthase. identified proteins are involved in different metabolic pathway, such as; energy metabolism, photosynthesis, redoxin, translation, transcription, signaling, osmolite synthesis, cell wall and replication factor.
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عنوان ژورنال:
علوم گیاهان زراعی ایرانجلد ۴۳، شماره ۱، صفحات ۱۳۹-۱۵۰
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